Multiplex-polymerase chain reaction analysis using short tandem repeats for detection of SRY and microdeletion of the azoospermia factor (AZF) region. Samples (p1, p2, and p3) of SRY-negative 46,XX testicular disorders of sex development show complete deletion of the AZFb and AZFc regions. Lanes for the DNA ladder, blank, and female negative controls were loaded and electrophoresed (data not shown).

m, male positive control; p, patient; ZFX, zinc finger protein, X-linked.