Fig. 2. Differential banding patterns of the 10 identified DEGs. The arrows with the gene name indicate the up-regulated (A) or down-regulated (B) DEGs in the Dex-treated cells compared to untreated control cells. Osteoblastic MC3T3-E1 cell line was treated with 1×PBS (Con) or 1 μM Dex, and then cultured for 2 days. Annealing control primer-based RT-PCR was performed on the total RNAs isolated from the treated cells, and RT-PCR products were resolved on 2% agarose gels and visualized by staining with ethidium bromide. DEG, differentially expressed gene; Con, control; Dex, dexamethasone; PBS, phosphate-buffered saline; RT-PCR, reverse-transcription polymerase chain reaction.
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