Fig. 3. Validation of the mRNA expression levels of the 10 DEGs in the Dex-treated MC3T3-E1 cells by quantitative real-time RT-PCR. Quantitative RT-PCR was performed using the total RNA samples. The mRNA expression level for each gene was quantified and the data represents the relative ratio of Dex-treated samples (Dex) compared to 1×PBS-treated samples (Con) in MC3T3-E1 cells. The Dex-insensitive housekeeping gene, Gapdh, was used for plotting the relative standard curve (internal control). Each experiment was repeated three times. *P<0.05, **P<0.01 vs. Control. DEG, differentially expressed gene; Dex, dexamethasone; RT-PCR, reverse-transcription polymerase chain reaction; Dex, dexamethasone; PBS, phosphate-buffered saline; Con, control.
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