Fig. 4. Comparison of the bone mineral density and the mRNA expression levels of the 10 DEGs between the sham and OVX mice. (A) Whole-body BMD was measured in the OVX or sham-operated control (Sham) mice (10 mice per group) using on-board PIXI-mus software for small animals and adjusted for the mouse body weight. Results are expressed as a percentage change of whole-body BMD adjusted for body weight (mean±standard deviation). **P<0.01 vs. Sham mouse group. (B) Quantitative reverse-transcription-PCR was performed using the total RNAs from the two mouse groups. The mRNA expression level for each gene was quantified and the data represents the relative ratio of OVX mice compared to Sham mice. The housekeeping gene, Gapdh, was used for plotting the relative standard curve (internal control). Each experiment was repeated three times. *P<0.05, **P<0.01 vs. Sham mouse group. DEG, differentially expressed gene; BMD, bone mineral density; OVX, ovariectomized.
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